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390953
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B94499PTO
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| Price |
$ 1,666.49
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$ 3,649.12
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| Product ID | ||
| Short Description | SDS-MW Analysis Kit | Fast Glycan Labeling and Analysis Kit |
| Long Description | The SDS-Gel MW Analysis Kit is designed for use on the PA 800 Plus and is used for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product. This kit provides reagent volumes for analysis of 100 samples and includes: • Separation Capillary, 67 cm x 50 µm ID, Bare-Fused Silica, (2) • SDS Gel Separation Buffer, 140 mL • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL • MW Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL • Acidic Wash Solution, 0.1 N HCl, 100 mL • Basic Wash Solution, 0.1 N NaOH, 100 mL | The Fast Glycan Labeling and Analysis kit is designed for use on the PA 800 Plus and is used to rapidly label glycoprotein released N-Glycans with APTS followed by the effective removal of the excess dye using a magnetic bead-based clean-up. The labeled glycans are separated by high-resolution capillary electrophoresis utilizing laser-induced flourescence (LIF) detection and the HR-NCHO separation gel. Glycan identification is determined based on their normalized electrophoretic mobility referenced against pre-determined GU values using the appropriate bracketing standard. This kit provides reagent volumes for analysis of 100 samples and includes: • M1, 22 mL • D1, 0.05 mL x 5 vials • D2, 250mM/50 µL dried x 5 vials • D3, 1.5 mL x 1 vial • D4, 1.5 mL x 2 vials • L5, 5 mg x 1 vial • L6, 5 mg x 5 vials • GU Ladder, 5 mg x 1 vial • IST (Internal Standard), 5 mg x 1 vial • BST (Bracketing Standard), 10 pmol x 1 vial • HR-NCHO Separation Gel, 56ml • Magnetic Separator, 1 unit • Pre-Assembled Cartridge (P/N A55625), 1 unit |
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