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390953
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5308349
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477600
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| Price |
$ 1,666.49
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$ 5,863.60
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$ 2,295.94
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| Product ID | |||
| Short Description | SDS-MW Analysis Kit | Nucleic Acid Extended Range Gel Multi Pack | Carbohydrate Labeling & Analysis Kit |
| Long Description | The SDS-Gel MW Analysis Kit is designed for use on the PA 800 Plus and is used for the separation and sizing of protein–SDS complexes using a replaceable gel matrix. The gel is formulated to provide an effective protein sieving range of approximately 10 to 225 kDa. Within this size range, the logarithm of protein molecular mass is linear with its reciprocal electrophoretic mobility, allowing the molecular weight of an unknown protein to be estimated from a standard curve of known protein sizes. This chemistry can also be used to effectively quantify the amount of protein and to determine the purity of a protein product. This kit provides reagent volumes for analysis of 100 samples and includes: • Separation Capillary, 67 cm x 50 µm ID, Bare-Fused Silica, (2) • SDS Gel Separation Buffer, 140 mL • SDS Sample Buffer, 100 mM Tris-HCl, pH 9.0/1% SDS, 50 mL • MW Sizing Standard (10 to 225 kDa), 16 mg/mL, 100 µL • Internal Standard, 10 kDa protein, 5 mg/mL, 0.4 mL • Acidic Wash Solution, 0.1 N HCl, 100 mL • Basic Wash Solution, 0.1 N NaOH, 100 mL | A 4-pack of the Nucleic Acid Extended Range Gel (4 x 140 mL), which is found in the RNA 9000 Purity & Integrity kit. This gel pack allows for the purchasing of only the gel and the ability to use a dye and ladder of your choosing. | The Carbohydrate Labeling and Analysis Kit is designed for use on the PA 800 Plus and contains the reagents, buffers, and capillaries required to label, separate, and quantify oligosaccharides and monosaccharides released from glycoproteins. After enzymatic or chemical release, sugars are labeled with a fluorophore (APTS) at the reducing termini by reductive amination. The stoichiometry of labeling is such that only one APTS molecule is attached to each molecule of oligosaccharide. These highly charged and fluorescent oligosaccharides are easily resolved in an electric field and detected by laser-induced fluorescence (LIF) detection. This kit provides reagent volumes for analysis of 100 samples and includes: • Carbohydrate Separation Buffer, 56 mL • N-CHO Coated Capillary, 2 units • Labeling Reagents, 1 vial • Labeling Dye (APTS), 4 x 5 mg • Labeling Dye Solvent, 1 mL • Glucose Ladder Standard, 50 mg • Quantitation/Mobility Marker (Maltose), 0.18 mg • APTS-M (monosaccharide-grade), 20 mg |
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